Composite

Part:BBa_K4011016:Design

Designed by: Ziteng Feng   Group: iGEM21_LINKS_China   (2021-10-21)


pTEF1-SaACS2-tADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1987
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2412
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 2662


Design Notes

1. All codons were optimized for S. cerevisiae based on S. cerevisiae codon bias

2. BsaI restriction sites were added to the three basic parts used to construct pTEF1-SaACS2-tADH1 for Golden Gate assembly.

3. BsmB1 sites flank the composite part for further Golden Gate assembly construction.


Source

pTEF1 and tADH1 all come from S. cerevisiae, while SaACS2 comes from Salmonella enterica.

References