Composite
Part:BBa_K4011016:Design
Designed by: Ziteng Feng Group: iGEM21_LINKS_China (2021-10-21)
pTEF1-SaACS2-tADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2426
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1987
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2412
Illegal BsaI.rc site found at 205
Illegal BsaI.rc site found at 2662
Design Notes
1. All codons were optimized for S. cerevisiae based on S. cerevisiae codon bias
2. BsaI restriction sites were added to the three basic parts used to construct pTEF1-SaACS2-tADH1 for Golden Gate assembly.
3. BsmB1 sites flank the composite part for further Golden Gate assembly construction.
Source
pTEF1 and tADH1 all come from S. cerevisiae, while SaACS2 comes from Salmonella enterica.